Separation of Hemoglobin from Bovine Serum Albumin
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This paper describes an experiment that used the SDS-PAGE technique to separate and determine the molecular weight of protein mixtures containing a hemoglobin and bovine serum albumin mixture of different dilutions. The paper also reviews how the experiment separated hemoglobin from bovine serum albumin using the ion exchange chromatography technique, using a DEAE cellulose column pre-equilibrated with Histidine buffer or a CM cellulose column pre-equilibrated with MES buffer. The paper outlines the methods and results of the study and concludes with a summary of the findings. The paper includes graphs and charts.
From the Paper:"Proteins are large molecules with polar and ionizing groups on their surface. Usually the pH affects the polar charges on the molecule, which causes the proteins to move to its oppositely charged electrode or remain stationary, depending on the buffer chosen. Many different electrophoretic systems have been used in the effective separation of protein mixtures. Two of these techniques are the SDS-PAGE technique which stands for Sodium Dodecyl Sulfate - Poly Acrylamide Gel Electrophoresis technique and the Ion-Exchange Chromatography (IEC) technique.
"The SDS-PAGE technique is often used to determine the molecular weight of a protein. One of the components used in this technique is the chemical substance SDS which stands for Sodium Dodecyl Sulfate. The substance SDS is used in this technique due to some useful characteristics because of its chemical structure. For instance, the hydrophobic tail of dodecyl sulfate interacts strongly with polypeptide chains and it also has two negative charges which collectively overwhelm or swamp any intrinsic charge of the protein."
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- Cruse, J & Lewis, R 1995, Illustrated Dictionary of Immunology, CRC Press Inc., Boca Raton, FL.
- Delves, P & Roitt, I 1999, Encyclopedia of Immunology, 2nd edn, Academic Press Inc., San Diego.
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- Kondo, A & Fukuda, H 1998, 'Effects of Adsorption Conditions on Kinetics of Protein Adsorption and Conformational Changes at Ultra ne Silica Particles', Journal of Colloid and Interface Science, vol. 198, no. 1, pp. 34-41, viewed 18 Apr 2011, http://www.sciencedirect.com/.
Cite this Research Paper:
Separation of Hemoglobin from Bovine Serum Albumin (2013, December 09) Retrieved August 02, 2021, from https://www.academon.com/research-paper/separation-of-hemoglobin-from-bovine-serum-albumin-153756/
"Separation of Hemoglobin from Bovine Serum Albumin" 09 December 2013. Web. 02 August. 2021. <https://www.academon.com/research-paper/separation-of-hemoglobin-from-bovine-serum-albumin-153756/>