Isolation and Observation of Bacteria Using Pure Culture Methods and Gram Staining
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This paper reviews an experiment on the isolation and observation of bacteria using pure culture methods and gram staining. The paper explores the streak plate method, spread plate method and the pour plate method techniques that produce these pure cultures, and then identifies the isolated bacteria using the Gram staining technique. The paper discusses the methods and results and explains how this experiment proved that cell shape does not decide Gram stain reaction. The paper also highlights the importance of all steps in the Gram stain, and the effect of omission of each step of the Gram stain on each of the bacteria.
From the Paper:"Vital to the development of microbiology during its 'golden age' was the advance inculturing techniques which enabled the isolation of bacterial colonies and the production of pure cultures of specific microorganisms.The study of pure cultures made it possible to determine the properties of a particular organism, such as its metabolic characteristics or its disease causing ability.Moreover, it also opened up the possibility of classifying microorganisms, on the basis of thecharacteristics they display in pure culture (Hogg, 2005). By definition, a pure culture is a population derived from an individual cell of a single species. Pure culture should always be axenic which means that it should be devoid of other, contaminating sorts of microorganisms (Starr, 1981). If they are not axenic then the results would be unreliable due to cross-contamination by another microorganism.
"The bacterial colonies that grow on a solid culture medium such as an agar plate or even from a broth culture are obtained in order to produce pure cultures. There are several techniques that are used to produce these pure cultures namely, the streak plate method, the spread plate method and the pour plate method."
Sample of Sources Used:
- Aneja, K.R. (2003). Methods of obtaining pure cultures of microorganisms. In Experiments in microbiology, plant pathology and biotechnology, 4th edn, p. 195.New Delhi: New Age International Publishers.
- Heritage, J., Evans, E.G.V. and Killington, R.A. (1996). Isolation, classification and identification of microbes. In Introductory microbiology, p. 138. Cambridge: Cambridge University Press.
- Hogg, S. (2005). Microbial nutrition and cultivation. In Essential microbiology, pp. 84-86. West Sussex: John Wiley & Sons Ltd.
- Starr, M.P. (1981). In The Prokaryotes: a handbook on habitats, isolation, and identification of bacteria, p. 155. New York: Springer-Verlag.
- Vasanthakumari, R. (2009). Stains and staining techniques. In Practical Microbiology, p.23. New Delhi: BI Publications Pvt Ltd.
Cite this Research Paper:
Isolation and Observation of Bacteria Using Pure Culture Methods and Gram Staining (2013, December 09) Retrieved September 22, 2019, from https://www.academon.com/research-paper/isolation-and-observation-of-bacteria-using-pure-culture-methods-and-gram-staining-153755/
"Isolation and Observation of Bacteria Using Pure Culture Methods and Gram Staining" 09 December 2013. Web. 22 September. 2019. <https://www.academon.com/research-paper/isolation-and-observation-of-bacteria-using-pure-culture-methods-and-gram-staining-153755/>