Functional Investigation of QPT Promoters Research Paper by Rifkhan
Functional Investigation of QPT Promoters
A review of a study on QPT promoters, using a small gene family from Nicotiana.
# 153753 | 4,766 words | 14 sources | APA | 2012 |
Published on Dec 06, 2013 in Biology (Biotechnology) , Agricultural Studies (General)
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This paper reviews a study that analyzes whether the QPT1 and QPT2 promoters are capable of carrying out their functions as promoters in different plant species. Furthermore, the paper looks at how this experiment explores the strength of the QPT2 promoter against the CaMV35S promoter, the effects of wounding and the effects of the treatments on different tissues like leaves and roots. The paper outlines and discusses the methods used and results gained, and concludes with a summary of the findings of the study.
Materials and Methods
Materials and Methods
From the Paper:"Each of the four plants species N. tabacum, Daucus carota (carrot), Petunia parodii (petunia) and Capsicum annum (chilli) were transformed with four different Agrobacterium rhizogenes strains. One of these strains had the wild type with no promoter added, while each of the other three strains had a CaMV35S, NQPT1 or NQPT2 promoter added. These plant tissues were agro-infected with the Agrobacterium rhizogenes suspensions and then incubated at 25oC for three days, after which, the ex-plant discs were transferred into fresh agar dishes M+S nutrient media containing 500mg/L cefotaxime. Three days later a second transfer to M+S cx medium was done. Five weeks after initial infection, transgenic roots were observed and the number of roots for both ex-plant species was counted. Using forceps, 20 roots from each plant species were put into Petri dishes with 2mL X-Gluc staining buffer and incubated overnight at 37oC. After the roots were stained the buffer was replaced with 70% ethanol. The number of roots that stained blue out of the 20 roots examined in all species was recorded (Song et al., 2012)."
Sample of Sources Used:
- Akhtar S., Briddon R.W. and Mansoor S. (2011). Reactions of Nicotiana species to inoculation with monopartite and bipartite begomoviruses. Virology Journal 8: 475.
- Akramian M., Tabatabaei S.M.T. and Mirmasoumi M. (2008). Virulence of different strains of Agrobacterium rhizogenes on genetics transformation of four Hyoscyamus species. American-Eurasian journal of agriculture and environmental science 3: 759-763.
- Cane K.A., Mayer M., Lidgett A.J., Michael A.J. and Hamill J.D. (2005). Molecular analysis of alkaloid metabolism in AABB vs. aabb genotype Nicotiana tabacum in response to wounding of aerial tissues and methyl jasmonate treatment of cultured roots. Functional Plant Biology 32: 305-320.
- Carmine A.A., Brogden R.N., Heel R.C., Speight T.M. and Avery G.S. (1983). Cefotaxime A review of its Antibacterial Activity, Pharmacological Properties and Therapeutic Use. Drugs 25: 223-289.
- Chase M.W., Knapp S., Cox A.V., Clarkson J.J., Butsko Y., Joseph J., Savolaineni V. and Parokonny A.S. (2003). Molecular systematics, GISH and the origin of hybrid taxa in Nicotiana (Solanaceae). Annals of Botany 92: 107-127.
Cite this Research Paper:
Functional Investigation of QPT Promoters (2013, December 06) Retrieved May 28, 2023, from https://www.academon.com/research-paper/functional-investigation-of-qpt-promoters-153753/
"Functional Investigation of QPT Promoters" 06 December 2013. Web. 28 May. 2023. <https://www.academon.com/research-paper/functional-investigation-of-qpt-promoters-153753/>