Electrophoresis of DNA Fragments
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This paper describes an experiment that studies the movement of charged molecules such as amino acids and nucleic acids, using two types of electrophoresis techniques; paper electrophoresis and agarose gel electrophoresis. The paper outlines the methods used in the study and the results gained. The paper concludes that the migration rate of DNA fragments is inversely proportional to fragment size.
From the Paper:"Paper electrophoresis was introduced in the 1950s as a separation technique for proteins and is carried out on paper strips soaked in buffer. Prior to the start of the electrophoretic run, the paper is saturated with the running buffer and placed in a tank. The sample is then applied as a spot or a line, and an electric field is applied. After the separation has proceeded for a sufficient time the paper is removed and dried. The separated components are then located by color or by fluorescence under UV light or by staining in a dye (Mikkelsen & Corton, 2004).
"Agarose gels which are made from purified agar and buffer solutions are required for agarose gel electrophoresis. This type of electrophoresis is very reliable in analyzing DNA and is hence used in this experiment to determine the sizes of nucleic acid fragments and this is usually carried out at a neutral pH. The larger the nucleic acid molecules the slower they travel through the gel compared to the smaller molecules which travel faster."
Sample of Sources Used:
- Buxbaum, E 2010, Biophysical Chemistry of Proteins: An Introduction to Laboratory Methods, Springer Science, New York, p. 67.
- Mikkelsen, SR & Corton, E 2004, Bioanalytical chemistry, John Wiley & Sons, New Jersey, p.171.
- Vijayakumar, T, Koshy, AS, Prabeesh, E & Rema, AK 2008, Biochemistry for Nursing and Healthcare, BI Publications Pvt Ltd, New Delhi, p.16.
Cite this Research Paper:
Electrophoresis of DNA Fragments (2013, December 06) Retrieved October 21, 2020, from https://www.academon.com/research-paper/electrophoresis-of-dna-fragments-153752/
"Electrophoresis of DNA Fragments" 06 December 2013. Web. 21 October. 2020. <https://www.academon.com/research-paper/electrophoresis-of-dna-fragments-153752/>