Cloning and Expression of Alpha-Amylase Gene
A report on a research project that was on the cloning and expression of the alpha-amylase gene from Bacillus subtilis, using Escherichia Coli as the host.
# 153729 | 3,338 words | 17 sources | APA | 2013 |
Published on Nov 21, 2013 in Biology (Molecular and Cell) , Biology (Biotechnology) , Hot Topics (Cloning)
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This research project studies the cloning and expression of an industrially important enzyme by amplifying the amyE gene that codes for -amylase enzyme in Bacillus subtilis. The paper explains that genomic DNA from B. subtilis was inserted into a vector and expressed into E. coli cells, allowing induction of IPTG and protein secretion. The paper details the results of this experiment and includes tables, graphs and photographs. The paper concludes with a summary of the results and several appendices.
Results and Discussion
Results and Discussion
From the Paper:"Amylases are among the most important industrial enzymes, accounting for about 30% of the world's total enzyme production. They have numerous industrial applications extending from general products such as detergents, paper, beer and textiles to clinical biology, health science and even the pharmaceutical industry (Maarel et al., 2002). -Amylases (E.C.126.96.36.199.) are starch-degrading enzymes that catalyze the hydrolysis of internal -1,4-O-glycosidic bonds in polysaccharides such as starch, to produce fine diverse products including glucose, dextrin and maltose (Bordbar et al., 2005).
" -amylase can be derived from sources such animals, plants and microbes. However, microbial sources such as fungi and bacteria are widely used in industrial production due to their high consistency and production capacity, lower production cost, faster production, non-pathogenic nature and increased product purity and stability (Burhan et al. 2003). Among bacteria, the Bacillus sp. is widely used in thermo-stable -amylase production to meet industrial needs. B. subtilis, B. stearothermophilus, B. licheniformis and B. amyloliquefaciens are widely used in the commercial production of -amylase for various applications (Sivaramakrishnan et al., 2006)."
Sample of Sources Used:
- Bordbar, AK, Omidiyan, K & Hosseinzadeh, R 2005, 'Study on interaction of a-amylase from Bacillus subtilis with cetyltrimethylammonium bromide', Colloids and Surfaces B: Biointerfaces, vol. 40, no. 1, pp. 67-71.
- Burhan, A, Nisa, U, Gokhan, C, Omer, C, Ashabil, A & Osman, G 2003, 'Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6', Process Biochemistry, vol. 38, no. 10, pp. 1397-1403.
- Cornelis, P 2000, 'Expressing genes in different Escherichia coli compartments', Current Opinion in Biotechnology, vol. 11, no. 5, pp. 450-454.
- Kakeshita, H, Kageyama, Y, Ara, K, Ozaki, K & Nakamura, K 2011, 'Propeptide of Bacillus subtilis amylase enhances extracellular production of human interferon- in Bacillus subtilis', Applied Microbiology and Biotechnology, vol. 89, no. 5, pp. 1509-1517.
- Kunst, F, Ogasawara, N, Moszer, I, Albertini, AM, Allonii, G, Azevedo, V, et al. 1997, 'The complete genome sequence of the gram positive bacterium Bacillus subtilis', Nature, vol. 390, pp. 249-267.
Cite this Research Paper:
Cloning and Expression of Alpha-Amylase Gene (2013, November 21) Retrieved February 18, 2020, from https://www.academon.com/research-paper/cloning-and-expression-of-alpha-amylase-gene-153729/
"Cloning and Expression of Alpha-Amylase Gene" 21 November 2013. Web. 18 February. 2020. <https://www.academon.com/research-paper/cloning-and-expression-of-alpha-amylase-gene-153729/>