Cloning of Thermo-Stable Alpha-Amylases Comparison Essay by Rifkhan

Cloning of Thermo-Stable Alpha-Amylases
A comparison of thermo-stable Alpha-amylase products cloned in Bacillus stearothermophilus and Bacillus subtilis.
# 153726 | 1,362 words | 13 sources | APA | 2013 | SA
Published on Nov 19, 2013 in Biology (Biotechnology)

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This paper discusses the cloning of thermo-stable Alpha-amylases in both Bacillus stearothermophilus and Bacillus subtilis and compares the biochemical quality of the Alpha-amylase enzyme produced from each bacterial strain. The paper finds that Alpha-amylase from both Bacillus stearothermophilus and Bacillus subtilis can be used in almost all industrial applications due to their high thermal, pH and chemical stabilities. The paper reveals, however, that Bacillus stearothermophilus is much more ideal for industry than Bacillus subtilis, as it is much more active in extreme temperatures and requires less metal ions for functioning. The paper notes that this is very important in most food production industries such as glucose , alcohol and beer.

Process of Cloning and Expression of -Amylase Gene for Both Bacterial Species
Comparison of the Quality of Alpha-Amylase Product from Bacillus Stearothermophilus and Bacillus Subtilis

From the Paper:

"The enzyme Alpha-amylase accounts for about 30% of total enzyme production in the world and contributes to numerous industrial applications, extending from general products such as detergents, paper, beer and textiles to clinical biology, health science and even the pharmaceutical industry (cyclo-dextrin manufacture). This enzyme hydrolyses the internal (1,4) glycosidic linkages present between glucose molecules within the polysaccharide starch, eventually degrading starch into glucose, maltose and dextrin. Alpha-amylases are metallo-enzymes and hence need calcium ions for stability, functioning and structural integrity. Alpha-amylases are mainly obtained from animals, plants and microbes (Chi et al. 2009; Maarel et al. 2002).
"Microbial sources such as bacteria are most widely used to produce enzymes on a large scale. Among bacteria, the Bacillus species is selected for enzyme manufacture due to their high consistency and production capacity, lower production cost, faster production, non-pathogenic nature and increased purity and stability of the enzyme product.The bacterial species that are the focus of this article, Bacillus stearothermophilus and Bacillus subtilis, are specifically selected in industrial Alpha-amylase manufacture due to their ability to withstand extreme pH, temperatures, organic solvents and other denaturing agents and their well-defined protein secretion process."

Sample of Sources Used:

  • Aiba, S, Kitai, K & Imanaka, T 1983, 'Cloning and expression of thermostable -amylase gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis, Applied and Environmental Microbiology, vol. 46, no. 5, pp. 1059-1065.
  • Asghar, M, Asad, MJ, Rahman, SU & Legge, RL 2007,'A thermostable -amylase from a moderately thermophilic Bacillus subtilis strain for starch processing', Journal of Food Engineering, vol. 79, pp. 950-955.
  • Chi, Z, Wang, F, Chi, Z, Yue, L, Liu, G & Zhang, T 2009, 'Bio-products from Auerobasidium pullulans, a biotechnologically important yeast, Applied Microbiology and Biotechnology, vol. 82, pp. 794-796.
  • Haki, GD & Rakshit, SK 2003, 'Developments in industrially important thermostable enzymes', Bio-resource Technology, vol. 89, no. 1, pp. 17-34.
  • Imanaka, T, Fuji, M, Aramori, I & Aiba, S 1982, 'Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis', The Journal of Bacteriology, vol. 149, pp. 824-830.

Cite this Comparison Essay:

APA Format

Cloning of Thermo-Stable Alpha-Amylases (2013, November 19) Retrieved December 07, 2021, from

MLA Format

"Cloning of Thermo-Stable Alpha-Amylases" 19 November 2013. Web. 07 December. 2021. <>