Advanced Technologies for Sequencing DNA Analytical Essay by Rifkhan

Advanced Technologies for Sequencing DNA
An examination of the advanced technologies available for rapid, large scale and cost-effective sequencing of genomes.
# 153724 | 1,353 words | 10 sources | APA | 2013 | SA
Published on Nov 19, 2013 in Biology (Genetics)

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The paper describes the conventional Sanger sequencing method and how it was highly unaffordable to produce enough sequencing to decipher even a comparatively small genome. The paper then looks at the first 'next-generation' sequencing (NGS) platform, the GS 20, that was introduced into the market in 2005, and explains the main principle behind NGS technologies. The paper considers the pros and cons of current NGS technologies in comparison to Sanger sequencing, and concludes that only time and inventiveness will establish the limits and the consequent abilities of this new paradigm. The paper includes an illustrated summary of some NGS techniques.

Conventional Sanger Sequencing Method and the NGS Technology Revolution
Pros and Cons of Current NGS Technologies in Comparison to Sanger Sequencing

From the Paper:

"While low-cost, revolutionizing computers and gadgets seem to dazzle our eyes every time we look around, a recent and fairly rapid progression in cost-effective genome analyses is drawing quite a lot of attention, the world over. Similar to electronics, the prices for rapid sequencing of genomes are in free fall, making deciphering the genetic code a simpler task "Over the last five years, the development of various 'next-generation' (NGS) or 'second generation' sequencing technologies has transformed large-scale sequencing, resulting in considerable cost reduction and greater volumes of genome sequence data, as compared to the conventional methods prevailing two years ago. NGS technologies could sequence up to a billion bases per day at low cost, leaving extensive genome sequencing in the reach of scores of researchers (Pop and Salzberg, 2008; Metzker, 2010; Nowrousian, 2010). This cost-effectiveness was attained by the implementation of automation and various innovative modifications of Sanger sequencing, instead of inventing entirely new procedures (Shendure et al., 2004). This article examines how this development has come about and what can these NGS technologies contribute to an enlightened future of scientific discoveries."

Sample of Sources Used:

  • Hall N. (2007). Advanced sequencing technologies and their wider impact in microbiology. The Journal of Experimental Biology 209: 1518-1525.
  • Hert D.G., Fredlake C.P. and Barron A.E. (2008). Advantages and limitations of next-generation sequencing technologies: A comparison of electrophoresis and non-electrophoresis methods. Electrophoresis 29: 4618-4626.
  • Mardis E.R. (2008). The impact of next-generation sequencing technology on genetics. Trends in Genetics 24: 133-141.
  • Metzker M.L. (2010). Sequencing technologies - the next generation. Nature Reviews Genetics 11: 31-46.
  • Nowrousian M. (2010). Next-Generation Sequencing Techniques for Eukaryotic Microorganisms: Sequencing-Based Solutions to Biological Problems. Eukaryotic Cell 9: 1300-1310.

Cite this Analytical Essay:

APA Format

Advanced Technologies for Sequencing DNA (2013, November 19) Retrieved May 28, 2023, from

MLA Format

"Advanced Technologies for Sequencing DNA" 19 November 2013. Web. 28 May. 2023. <>