Papers on "Polymerase Chain Reaction-Derived Homeotic Genes" and similar term paper topics

Paper #104242 :: Polymerase Chain Reaction-Derived Homeotic Genes
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Describes a procedure to isolate the morphology-controlling homeotic genes of Caenorhabditis elegans.

Written in 2007; 1,800 words; 17 sources; APA; $ 57.95

Paper Summary:

This paper describes an experiment in which DNA was extracted from the organism Caenorhabditis elegans to be amplified and electrophoresed. The author reports that polymerase chain reaction (PCR) was used in the experiment to amplify the DNA and after electrophoresis was finished, a photograph of the gel was obtained. The author also explains that the migration distance of the bands was measured and a graph of the molecular weight of the bands was created. The paper states that this process allowed the isolation of homeobox genes, which are of great importance in many different organisms for patterning and segmentation.

Table of Contents:
Keywords
Abstract
Introduction
Materials & Methods
C. Elegans Preparation
RNAse Treatment and Protein Precipitation
DNA Precipitation, Drying and Rehydration
Preparation of PCR Reactions and Running of Reactions in Thermocycler
Homeobox PCR
Casting an Agrose Gel
Loading, Running and Staining the Agrose Gel
Results
Discussion
Figures and Tables

From the Paper:

"When it comes to unlocking the mysteries of organismal development, it all comes down to genes. However when genes are to be analyzed, a little is often not enough. This is where polymerase chain reaction (PCR) comes into play. PCR is a powerful and fast method, for which the ultimate desired outcome is the amplification and quantification of DNA "from a minimal initiating amount in vitro.
In order for PCR to occur, template DNA is first denatured. Annealing primers, or often degenerative primers which use every possible codons for unknown DNA sequences."

Tags: hox dna morphogenesis, migration distance, patterning segmentation.

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