Determination of Antibody Titre of Rabbit Anti-Ferretin Antibody Using Enzyme Linked Immunosorbent Assay
A practical attempt to determine the endpoint titre of rabbit anti-ferritin antibody by using the Enzyme Linked Immunosorbent Assay.
# 147819 | 1,270 words | 11 sources | APA | 2011 |
Published on Jul 19, 2011 in Biology (General) , Chemistry (Biochemistry) , Medical and Health (Medical Studies)
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This paper describes an experiment performed in order to determine the minimum concentration of rabbit anti-ferritin antibody by using the Enzyme Linked Immunosorbent Assay. The calculations and results are presented in a table and are accompanied by figures for clarity. The methodology and possibility of error is discussed. Finally, the paper considers the medical and economic implications of these findings.
From the Paper:"During this lab, an important biochemical technique was used. Enzyme Linked Immunosorbent Assay (ELISA) can be described as one of the most important methods based on the interactions between antibody and antigen (Pye, 2010). An antigen is capable of causing an immune response and react with the products of this response. A molecule that is antigenic may possess several epitopes. Epitopes are antigenic determinants that can bind with an antibody. An antibody recognizes antigens and prevents the antigens from causing damage. The ELISA involves the binding of antibodies to antigen. A primary antibody recognizes the antigen, and a secondary antibody binds to the primary antibody (Chapel et al, 1999). This secondary antibody has an enzyme covalently linked and can interact with a chromogenic or fluorogenic substrate (Pye, 2010).
"A brief history of the need for and discovery of ELISA should be given. The precursor to the ELISA was the radioimmunoassay. This process involved radioactively tagging either the antigens or antibodies under observation. The radiation served as the indicator instead of colorimetric enzyme-substrate reactions (Yalow and Berson, 1960). In 1971, Peter Perlmann, Eva Engvall, Anton Schuurs, and Bauke van Weemen formulated the means to accomplish ELISA."
Sample of Sources Used:
- Pye, David. (2010). Medical Biochemistry Methods Book. United Kingdom
- Chapel, Helen, Haeney, Mansel, Misbah, Siraj, and Snowden, Neil. (1999). Essentials of Clinical Immunology. Oxford: Blackwell Science Ltd
- Heath, Stephen. (2010). Clinical Genetics and Immunology: Level 5 Module. United Kingdom
- Blake, M. S., Johnston, K.H., Russell-Jones, G. J., and Gotschlich, E.C.. (1984). A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. United States of America: Elsevier Inc.
- Ong, D. S., Wang, L. Zhu, Y., Ho, B., and Ding, J.L. (2005). The response of ferritin to LPS and cute phase of Pseudomonas infection. Singapore: Sage Publications
Cite this Descriptive Essay:
Determination of Antibody Titre of Rabbit Anti-Ferretin Antibody Using Enzyme Linked Immunosorbent Assay (2011, July 19) Retrieved May 20, 2013, from http://www.academon.com/descriptive-essay/determination-of-antibody-titre-of-rabbit-anti-ferretin-antibody-using-enzyme-linked-immunosorbent-assay-147819/
"Determination of Antibody Titre of Rabbit Anti-Ferretin Antibody Using Enzyme Linked Immunosorbent Assay" 19 July 2011. Web. 20 May. 2013. <http://www.academon.com/descriptive-essay/determination-of-antibody-titre-of-rabbit-anti-ferretin-antibody-using-enzyme-linked-immunosorbent-assay-147819/>