Abstract This paper defines the term cytotoxicity and then presents an overview and critique of eye cytotoxicity tests: agar diffusion method; fluorscein leakage test; neutral-red uptake test; MTT-based cytotoxicity assay.
From the Paper "This research paper provided an overview and critique of the agar diffusion method, the fluorescein leakage test, the neutral-red uptake test and the MTT-based cytotoxicity assay. Cytotoxicity refers to a toxic effect at the cellular level such as altered cellular membrane permeability death or enzymatic inhibition. A toxic material is one that releases a chemical that kills cells directly or indirectly. Cell culture methods are used to evaluate cytoxicity bio compatibility and functioning of materials in the in vivo environment. Testing procedures are used for..."
Abstract This paper discusses the various forms of chemotherapy. The paper describes the different possible side effects of chemotherapy, outlining short term and long term side effects.
From the Paper "Cancer is a disease of the body's cells. Cells in all the tissues and organs of the body constantly grow and divide to swap old and damaged cells and maintain the health of the body. Normally, all cells divide and reproduce themselves in a systematic and controlled manner. In cancer, however, some cells keep dividing without proper control, forming a lump (which is called a tumor). In leukemia, too many white blood cells are formed. Chemotherapy is the use of anti-cancer (cytotoxic) drugs to destroy cancer cells (including leukemia's and lymphomas). There are over 50 different chemotherapy drugs and some are given on their own, but often numerous drugs may be combined (this is known as combination chemotherapy). The type of treatment one are given for ones cancer depends on many things, particularly the type of disease one have, where in the body it started, what the cancer cells look like under the microscope and how far they have spread, if at all."
Scientific review of journal article, "Determination of Safety Levels of Horseradish Peroxidase-Iodide System to Human Gingival Keratinocytes and Fibroblasts in Vitro" .
Abstract This paper explains and reviews an article about the effects of the horseradish peroxidase-iodide system on metabolic activity, membrane permeability, and cell growth of gingival keratinocytes and fibroblasts in vitro. The paper provides background information on the subject of the article, details the results of the experiment under discussion, and explains the significance of the experiment in terms of future research on the subject.
From the Paper "Collectively, these results demonstrate that of the three studied cellular indicators the effects of the complete HRP-I system were first observed as decreased metabolic activity and cell growth. Keratinocytes cell membrane permeability was altered only with concentrations of iodine and hydrogen peroxide as high as 25mmol/L in the complete HRP system in growth medium. The highest production of free iodine was measured to be by the HRP-I system in buffer compared to the growth medium. This is due to the presence of oxidizable free amino acids and antioxidants in the growth media. The highest concentrations of the HRP-Iodine system components which did not have any significant detrimental effects on the metabolic activity and cell growth of gingival keratinocytes and fibroblasts with exposure time as long as 1 hour did not have detrimental effects on the metabolism of HGK and HGF cells if the exposure time did not exceed 5 minutes. The effects of the HRP system on cell growth and metabolic activity were observed at lower iodide and hydrogen peroxide concentrations than its effects on membrane permeability. Gingival fibroblasts were more prone to detachment than keratinocyte cell lines, but no differences in changes of growth or metabolic activities were observed between gingival fibroblasts and gingival keratinocytes."
Abstract This paper briefly summarizes and then reviews an article on a potential cancer therapy entitled "Exploiting T cell Receptor Genes for Cancer Immunotherapy." The paper explains that the article reviewed discussed various cancer treatment options with T cell receptor immunotherapy and then goes on to discuss the results and conclusions found in the article. The paper concludes that the article was well-written and informative.
From the Paper "Adoptive antigen-specific immunotherapy is a potential cancer therapy because immunocompetence is not required and lymphocyte specificity can be targeted against tumour-associated antigens. T cell receptor (TCR) genes isolated from antigen-specific T cells may be therapeutically used for antigen-specific immunotherapy. TCR gene therapy may provide an appealing strategy to develop antigen-specific immunotherapy. Examples of this type of therapy include retroviral TCR gene transfer, TCR gene modified lymphocytes, and TCR gene expression."
Tags: monoclonal, generic, reagents, surface, helper, functions, cytotoxicity, cytokine, production
