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The paper states that Tetrahymena are model organisms that are commonly used to study their feeding behavior over various environments and preys. They are common holotrichic ciliates easily grown in the laboratory, using pure liquid cultures. They acquire their nutrients by ingesting smaller microbes such as bacteria and fungi. The paper comments on the tests performed on Tetrahymena phagocytosis of Saccharomyces cerevisiae in the presence of different concentrations of aspirin. The paper records that after treatment, the cells were analyzed by flow cytometer via computer program, ColorCell software, when percent phagocytosis were identified. It was originally thought that aspirin would decrease the phagocytotic ability of Tetrahymena; however, the data concluded that aspirin does not alter this ability over a period of 4 minutes. The paper states that further analysis is necessary with longer treatment period and using various other drugs.
Materials and Methods
Materials and Methods
From the Paper:"Utilizing such power technology, we are now able to study the feeding behavior of our model organism, Tetrahymena sp., using FITC-labeled yeast and determine if the prey has been phagocytized. We will label Saccharomyces cerevisiae with FITC dye and detect the ingestion under different environments. So we would like to explore the following questions: How long does it take for Tetrahymena to ingest food? And, do different concentrations of aspirin affect Tetrahymena feeding behavior? "
Sample of Sources Used:
- p://www.facslab.toxikologie.uni-mainz.de/engl.%20Websites/zytometrie-engl.jspWiliams NE, et al. The actin gene ACT1 is required for phagocytosis, motility, and cell separation of Tetrahymena thermophila. Eukaryot Cell. 2006 Mar;5(3):555-67. http://en.wikipedia.org/wiki/Flow_cytometry
- The effects of aspirin and acetaminophen on tetrahymena pyriformis GL growth and macromolecular synthesis.Res Commun Chem Pathol Pharmacol. 1984 Aug;45(2):293-6.
- "Effects of Aspirin on Tetrahymena", M.Geary, Lab Manual, 2006, West Valley College.
Cite this Case Study:
Tetrahymena (2008, November 18) Retrieved January 30, 2015, from http://www.academon.com/case-study/tetrahymena-109125/
"Tetrahymena" 18 November 2008. Web. 30 January. 2015. <http://www.academon.com/case-study/tetrahymena-109125/>