Molecular Cloning

Paper Summary:

This paper describes an experiment designed to refute or support the hypothesis that using recombinant DNA technology allows for the cloning of specific DNA fragments. The paper then analyzes the results of the experiment and concludes that the hypothesis is supported by the fact that five colonies were created that contained the specific 2.0 DNA fragment, signaling that the bacteria contained the desired insert-vector molecule. The paper includes several tables, graphs and images.

Table of Contents:
Abstract
Introduction
Materials and Methods
Results
Figure: Photograph of UV Transilluminated Electrophoresed Gel of Plasmid DNA, Lambda bacteriophage DNA, Calf Thymus DNA, and Calf Kidney DNA
Table: Distance Migrated and Size of DNA Molecules for Standards, Calf DNA, and Plasmid DNA
Graph: Lambda Bacteriophage DNA Log Base 10 of Molecular Weight versus Distance Migrated
Figure: Picture of Electrophoresed Gel Containing Standards, HSHV Cut and Uncut, CHAV Cut and Uncut, and KDV Cut and Uncut
Table: Molecular Weights of Standards, CHAV, KDV, and HSHV and the Distance Traveled in Electrophoresed Gel by CHAV, KDV, and HSHV
Graph: The Standards from Graph 1 used to Compare Against to Discover the Molecular Weight of KDV Virus Fragments
Table: The Characteristics of the E. coli Strands on the Plates
Table: The Characteristics of the Streaked Samples from the Tranformation 4 Plate
Figure: Picture of Electrophoresed Gel containing Known Standards, Uncut DNA from Blue Colony, Cut with HinDIII DNA from Blue Colony, and 5 Cut DNA with HindIII from White Colonies
Table: The Molecular Weights and Distance Traveled by the Standards, the Blue Colony both Cut and Uncut, and the Five Cut White Colonies
Graph: The DNA Standards Molecular Weights Versus the Distance Migrated
Table: The Characteristics and Plasmid Names of the Different E. coli Strains Created
Calculations
Discussion

From the Paper:

"The purpose of the second part in the recombination of DNA was to isolate plasmid DNA from E. coli harboring pUC18 plasmids with -DNA fragment inserts and cut the DNA with HindIII. Bacteria from Transformation 4, both blue and white colonies, were to be taken for inoculation. The white colonies could not be obtained because they are satellite colonies that grow from enzyme excretion. The samples were obtained from already prepared liquid forms of the white colonies."

Sample of Sources Used:

• Kornberg, A., & Baker, T. (2005). DNA Replication.University Science Books.
• Leisner, S. & Shemshedini, L. (2009). Fundamentals of Life Sciene I Lab Honors.Toledo:
• Reddi, O.S. (2000). Recombinant DNA Technology. Allied Publishers

More papers on Molecular Cloning:

• Term Paper # 66769 : "Genetic Engineering: Cloning"
• Term Paper # 66916 : "Genetic Engineering: Cloning"
• Research Paper # 47069 : "Ethics and Technology"

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