This paper looks at an experiment in an enzyme purification lab for the purification of lysozyme.
Written in 2007; 2,117 words; 5 sources; APA; $ 66.95
Paper Summary:
The writer explains that in this experiment, lysozyme was purified from egg white solution into 24 fractions using gel column chromatography. The writer points out that the odd-numbered fractions were then assayed in order to determine the enzymatic activity of lysozyme, while the even-numbered fractions were assayed for total protein content, using the Bradford protein assay procedure. The writer notes that lysozyme is one of the most well-known enzymes, being abundant in mucus, tears, and albumen (commonly known as egg white).
The writer concludes that the purification of lysozyme from egg white was only somewhat successful since the estimated value of the molecular weight, 14.25 kDa, is practically the same as the known weight of 14.3 kDa. However, the specific activity, calculated at 400 units/mg, was significantly less than the expected specific activity of 25 000 units/mg of protein.
Outline:
Abstract
Introduction
Materials and Methods
Results
Calculations
Discussion
Conclusion
From the Paper:
"Lysozyme is itself composed of 129 amino acids, including four disulfide bridges and three sets of alpha helices. The enzyme's active site consists of a long groove that can hold as many as six polysaccharides. According to past experiments, specific activity of lysozyme from egg white is approximately 25 000 units/mg. This is the expected result for this experiment. However, one factor that influences the specific activity is the actual column and type of chromatography being used, since accuracy differs among the different types of chromatographies. Therefore, some are able to purify enzymes better than others, giving a more accurate specific activity. In addition to obtaining the specific activity, molecular weight of the enzyme can be determined by comparing the fraction in which most of the enzyme eluted with the fractionation range."
We have thousands of high-quality term papers, research papers, essays, book reports and dissertations on every topic. At AcaDemon, you can download those term papers to help you write yours! You can be sure that the term paper, essay, book report or research paper you download are top-quality, competitively priced and high-level work.
This Free Term Paper Abstract is a part of our Term Paper Library.Here you can purchase research papers, examples of essays, academic dissertations, articles, notes, analytical papers, book reports, stories and poems. We have thousands of persuasive, point-of-view, narrative, critical, compare and contrast and other types of essays in our Library. You can also find here Term papers on "Isolation and Purification of Lysozyme", Essays on "Isolation and Purification of Lysozyme", Research papers on "Isolation and Purification of Lysozyme", Student papers on "Isolation and Purification of Lysozyme", Book reports on "Isolation and Purification of Lysozyme", Dissertation on "Isolation and Purification of Lysozyme", Thesis on "Isolation and Purification of Lysozyme", Summary of paper on "Isolation and Purification of Lysozyme", Articles written on "Isolation and Purification of Lysozyme".
Look for more research papers, essays and book reports on