Paper Summary:

This paper describes the Gram stain technique and what it shows. The paper also defines acid-fast bacteria and explains the differences between prokaryotic and eukaryotic cells.

From the Paper:

"The Gram stain is named for the Danish bacteriologist who first devised it. It is usually the first test used to identify bacteria. The Gram stain is based on the ability of bacterial cell walls to retain crystal violet dye during solvent treatment. Bacterial cells with walls containing small amounts of peptidoglycan andlipopolysaccharide are Gram-negative, those containing relatively high amounts of peptidoglycan and lipopolysaccharide are Gram-positive. The Gram microorganisms which retain the crystal violet-iodine complex appear purple-brown under the microscope and are considered Gram-positive."

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