An description of the methods and results of an experiment in W. MacGinnis and R. Krumlauf's article, "Homeobox Genes and Axial Patterning."
Written in 2006; 1,639 words; 1 sources; APA; $ 53.95
Paper Summary:
This paper presents and describes an experiment in which DNA was extracted from C. Elagens to be amplified and electrophoresed. The paper discusses information retrieved from W. MacGinnis and R. Krumlauf's article, "Homeobox Genes and Axial Patterning." The paper focuses on discussing the methods and results for the experiment.
Table of Contents:
Materials and Methods
C. Elegan Preparation
RNAse Treatment and Protein Precipitation
DNA Precipitation, Drying and Rehydration
Preparation of PCR Reactions and Running of Reactions in Thermocycler
Homeobox PCR
Casting an Agarose Gel
Loading, Running and Staining the Agarose Gel
Taking a Picture of the Gel
Results
Discussion
From the Paper:
"From the final results, it is apparent that the C. Elegans do possess homeobox genes. Since many of the bands had high molecular weight, they contained hundreds of base pairs, a sign indicating the presence of homeobox genes. The homeobox genes are of great importance as they encode proteins that tell the cells in the various segments of the developing embryo, what kind of structures to make (McGinnis W. and Krumlauf R.). There are however, many organisms that do not contain homeobox genes. Such organisms are not complex in a segmental way, such organisms that do not have segmentation have no need for genes to create structures that they no not need."
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