Isoproterenol's Effect on Chick Embryos

Paper Summary:

The paper hypothesizes that Isoproterenol will increase the heart rate of a chick embryo heart, in which the chick was incubated for approximately 48 hours, when topically applied. The author of the paper shows that the reasoning for this hypothesis is that Isoproterenol acts as a sympathetic stimulant by stimulating the beta receptors found in the heart, and these kinds of stimulants (hence the name stimulant) tend to speed up actions. Especially since this chemical mimics norepinephrine's effects, I deduced that this would speed up the heart rate. An experiment was performed on 48-hour chick embryos and the results are discussed in the paper.

From the Paper:

"Description: An egg was incubated for approximately 48 hours. I am approximating 48-hours based on the comparison of my embryo to pictures of the different stages. Once removed from the incubator, I made an incision around the circumference of the egg and removed the roof, exposing the interior. The embryo with all structures still attached- in situ- (yolk, egg white, etc.) was poured into a dish of saline solution. The egg remained in the same position for a couple of hours, blunt-end up, so that the embryo conveniently lay on top. The temperature of the water was measured as 32?C and the normal heart rate of the chick was recorded as 76bpm."

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