Horseradish Peroxidase

Paper Summary:

This paper explains and reviews an article about the effects of the horseradish peroxidase-iodide system on metabolic activity, membrane permeability, and cell growth of gingival keratinocytes and fibroblasts in vitro. The paper provides background information on the subject of the article, details the results of the experiment under discussion, and explains the significance of the experiment in terms of future research on the subject.

From the Paper:

"Collectively, these results demonstrate that of the three studied cellular indicators the effects of the complete HRP-I system were first observed as decreased metabolic activity and cell growth. Keratinocytes cell membrane permeability was altered only with concentrations of iodine and hydrogen peroxide as high as 25mmol/L in the complete HRP system in growth medium. The highest production of free iodine was measured to be by the HRP-I system in buffer compared to the growth medium. This is due to the presence of oxidizable free amino acids and antioxidants in the growth media. The highest concentrations of the HRP-Iodine system components which did not have any significant detrimental effects on the metabolic activity and cell growth of gingival keratinocytes and fibroblasts with exposure time as long as 1 hour did not have detrimental effects on the metabolism of HGK and HGF cells if the exposure time did not exceed 5 minutes. The effects of the HRP system on cell growth and metabolic activity were observed at lower iodide and hydrogen peroxide concentrations than its effects on membrane permeability. Gingival fibroblasts were more prone to detachment than keratinocyte cell lines, but no differences in changes of growth or metabolic activities were observed between gingival fibroblasts and gingival keratinocytes."

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