A presentation of the methods and results of an experiment to separate a mixture of two dyes by chromatography.
Written in 2007; 1,135 words; 5 sources; MLA; $ 39.95
Paper Summary:
This paper presents an experiment in which two types of chromatography were used to separate a mixture of two dyes, methylene blue and fluorescein. The materials and methods that were used for the experiment are discusses as well as the results that were obtained. The results are presented in a table form.
Table of Contents:
Introduction
Materials and Methods
Results
Discussion
Additional Experiments
Appendix
From the Paper:
"Gel permeation chromatography is a type of size exclusion chromatography (SEC), where the mobile phase is an organic solvent and separation occurs based on size. (Davies, Johnson, 2007). It is used to determine the molecular weights of organic and biological molecules, such as nucleic acids and proteins. The stationary phase is a gel medium, (e.g. agarose, polyacrylamide, or dextran) and elution occurs under low pressure. The molecules of interest are often further separated based on charge, acidity/bascitiy and affinity to particular molecules. This technique is efficient because all the samples can be simultaneously loaded without deterring the elution process, or disrupting the biologically-active state of the different samples. (Davies, Johnson, 2007)."
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